Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in <em>Caenorhabditis elegans</em>
نویسندگان
چکیده
منابع مشابه
Subcellular localization of the hypusine-containing eukaryotic initiation factor 5A by immunofluorescent staining and green fluorescent protein tagging.
Eukaryotic initiation factor 5A (eIF-5A) is the only protein in nature that contains hypusine, an unusual amino acid residue formed posttranslationally by deoxyhypusine synthase and deoxyhypusine hydroxylase. Although the eIF-5A gene is essential for cell survival and proliferation, the precise function and localization of eIF-5A remain unclear. In this study, we have determined the subcellular...
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متن کاملProtein Subcellular Localization Prediction
ORGANIZATION. Section 1. We first provide the motivation for prediction of protein subcellular localization sites, as well as discuss changes being brought about by progress in proteomics. Section 2. After that, we describe the biology of protein subcellular location. In particular, we explain the principle of protein sorting signals. Section 3. Then we present several experimental techniques f...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2018
ISSN: 1940-087X
DOI: 10.3791/57914